Scaling algae cultivation from laboratory conditions to industrial production scale is a complex process that requires careful consideration of biological, technical, and operational factors. This guide provides practical insights into scaling with Lgem’s photobioreactors (PBRs), addressing common challenges and strategies to ensure efficient and stable production at larger scales. 1. Key steps in transitioning from lab-scale to pilot-scale to industrial-scale cultivation Environmental control variations Lab environments offer high control over culture conditions such as temperature, pH, and light and nutrient availability. Scaling up introduces environmental variability, especially in outdoor systems subject to seasonal and daily variations. To mitigate this,